Identification and Validation of Reliable Reference Genes for Gene Expression Studies in Koelreuteria paniculata.

ABSTRACT: RT-qPCR is considered a rapid and reliable technique for analyzing gene expression. This technique is commonly used to analyze the expression of various genes at diverse transcriptional levels in different samples. However, few studies have characterized ornamental Koelreuteria species for reliable reference genes. In this study, eight reference genes were evaluated as controls in RT-qPCR with SYBR green to quantify gene expression in different Koelreuteria paniculata samples. All selected reference genes showed a broad range of C_t values in all samples, which was supportive of their variable expression. Our results showed significant variation in the stable expression of K. paniculata genes. Sample data, analyzed using geNorm, NormFinder, and BestKeeper, sh

SUBMITTER: Gao K 

PROVIDER: S-EPMC9141280 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature