Mitochondrial Inhibition by Sodium Azide Induces Assembly of eIF2α Phosphorylation-Independent Stress Granules in Mammalian Cells.

ABSTRACT: Mitochondrial stress is involved in many pathological conditions and triggers the integrated stress response (ISR). The ISR is initiated by phosphorylation of the eukaryotic translation initiation factor (eIF) 2α and results in global inhibition of protein synthesis, while the production of specific proteins important for the stress response and recovery is favored. The stalled translation preinitiation complexes phase-separate together with local RNA binding proteins into cytoplasmic stress granules (SG), which are important for regulation of cell signaling and survival under stress conditions. Here we found that mitochondrial inhibition by sodium azide (NaN₃) in mammalian cells leads to translational inhibition and formation of SGs, as previously shown in yeast. Although mamma

SUBMITTER: Eiermann N 

PROVIDER: S-EPMC9142010 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature