Bronchial epithelium epithelial-mesenchymal plasticity forms aberrant basaloid-like cells in vitro.

ABSTRACT: Although epithelial-mesenchymal transition (EMT) is a common feature of fibrotic lung disease, its role in fibrogenesis is controversial. Recently, aberrant basaloid cells were identified in fibrotic lung tissue as a novel epithelial cell type displaying a partial EMT phenotype. The developmental origin of these cells remains unknown. To elucidate the role of EMT in the development of aberrant basaloid cells from the bronchial epithelium, we mapped EMT-induced transcriptional changes at the population and single-cell levels. Human bronchial epithelial cells grown as submerged or air-liquid interface (ALI) cultures with or without EMT induction were analyzed by bulk and single-cell RNA-Sequencing. Comparison of submerged and ALI cultures revealed differential expression of 8,247 protein cod

SUBMITTER: Uthaya Kumar DB 

PROVIDER: S-EPMC9142163 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature