Mitochondrial Calcium Disorder Affects Early Embryonic Development in Mice through Regulating the ERK/MAPK Pathway.

ABSTRACT: The homeostasis of mitochondrial calcium ([Ca²⁺]_mt) in oocytes plays a critical role in maintaining normal reproductive cellular progress such as meiosis. However, little is known about the association between [Ca²⁺]_mt homeostasis and early embryonic development. Two in vitro mouse MII oocyte models were established by using a specific agonist or inhibitor targeting mitochondrial calcium uniporters (MCU) to upregulate or downregulate [Ca²⁺]_mt concentrations. The imbalance of [Ca²⁺]_mt in MII oocytes causes mitochondrial dysfunction and morphological abnormity, leading to an abnormal spindle/chromosome structure. Oocytes in drug-treated groups are less likely to develop into blastocyst during in

SUBMITTER: Zhang L 

PROVIDER: S-EPMC9142297 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature