Yeast-based reference materials for quantitative metabolomics.

ABSTRACT: We introduce a new concept of yeast-derived biological matrix reference material for metabolomics research relying on in vivo synthesis of a defined biomass, standardized extraction followed by absolute quantification with isotope dilution. The yeast Pichia pastoris was grown using full control- and online monitoring fed-batch fermentations followed by fast cold methanol quenching and boiling ethanol extraction. Dried extracts served for the quantification campaign. A metabolite panel of the evolutionarily conserved primary metabolome (amino acids, nucleotides, organic acids, and metabolites of the central carbon metabolism) was absolutely quantified by isotope dilution utilizing uniformly labeled ¹³C-yeast-based internal standards. The study involved two independent laboratorie

SUBMITTER: Wasito H 

PROVIDER: S-EPMC9142427 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature