Procedure providing SI-traceable results for the calibration of protein standards by sulfur determination and its application on tau.

ABSTRACT: Quantitative proteomics is a growing research area and one of the most important tools in the life sciences. Well-characterized and quantified protein standards are needed to achieve accurate and reliable results. However, only a limited number of sufficiently characterized protein standards are currently available. To fill this gap, a method for traceable protein quantification using sulfur isotope dilution inductively coupled plasma mass spectrometry (ICP-MS) was developed in this study. Gel filtration and membrane filtration were tested for the separation of non-protein-bound sulfur in the protein solution. Membrane filtration demonstrated a better performance due to the lower workload and the very low sulfur blanks of 11 ng, making it well suited for high-purity proteins such as NIST S

SUBMITTER: Lemke N 

PROVIDER: S-EPMC9142460 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature