Dataset Information

Oxidative stress monitoring in iPSC-derived motor neurons using genetically encoded biosensors of H₂O₂.

ABSTRACT: Oxidative stress plays an important role in the development of neurodegenerative diseases, being either the initiator or part of a pathological cascade that leads to the neuron's death. Genetically encoded biosensors of oxidative stress demonstrated their general functionality and overall safety in various systems. However, there is still insufficient data regarding their use in the research of disease-related phenotypes in relevant model systems, such as human cells. Here, we establish an approach for monitoring the redox state of live motor neurons with SOD1 mutations associated with amyotrophic lateral sclerosis. Using CRISPR/Cas9, we insert genetically encoded biosensors of cytoplasmic and mitochondrial H₂O₂ in the genome of induced pluripotent stem cell (iPSC) lines. We demonstrate that the biosensors remain functional in motor neurons derived from these iPSCs and reflect the differences in the stationary redox state of the neurons with different genotypes. Moreover, we show that the biosensors respond to alterations in motor neuron oxidation caused by either environmental changes or cellular stress. Thus, the obtained platform is suitable for cell-based research of neurodegenerative mechanisms.

SUBMITTER: Ustyantseva E

PROVIDER: S-EPMC9142597 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Oxidative stress monitoring in iPSC-derived motor neurons using genetically encoded biosensors of H<sub>2</sub>O<sub>2</sub>.

Ustyantseva Elizaveta E Pavlova Sophia V SV Malakhova Anastasia A AA Ustyantsev Kirill K Zakian Suren M SM Medvedev Sergey P SP

Scientific reports 20220527 1

Oxidative stress plays an important role in the development of neurodegenerative diseases, being either the initiator or part of a pathological cascade that leads to the neuron's death. Genetically encoded biosensors of oxidative stress demonstrated their general functionality and overall safety in various systems. However, there is still insufficient data regarding their use in the research of disease-related phenotypes in relevant model systems, such as human cells. Here, we establish an appro ...[more]

PMID: 35624228

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Dataset Information

Oxidative stress monitoring in iPSC-derived motor neurons using genetically encoded biosensors of H₂O₂.

Publications

Oxidative stress monitoring in iPSC-derived motor neurons using genetically encoded biosensors of H<sub>2</sub>O<sub>2</sub>.

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Dataset Information

Oxidative stress monitoring in iPSC-derived motor neurons using genetically encoded biosensors of H2O2.

Publications

Oxidative stress monitoring in iPSC-derived motor neurons using genetically encoded biosensors of H<sub>2</sub>O<sub>2</sub>.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Oxidative stress monitoring in iPSC-derived motor neurons using genetically encoded biosensors of H₂O₂.