Oxidative stress monitoring in iPSC-derived motor neurons using genetically encoded biosensors of H₂O₂.

ABSTRACT: Oxidative stress plays an important role in the development of neurodegenerative diseases, being either the initiator or part of a pathological cascade that leads to the neuron's death. Genetically encoded biosensors of oxidative stress demonstrated their general functionality and overall safety in various systems. However, there is still insufficient data regarding their use in the research of disease-related phenotypes in relevant model systems, such as human cells. Here, we establish an approach for monitoring the redox state of live motor neurons with SOD1 mutations associated with amyotrophic lateral sclerosis. Using CRISPR/Cas9, we insert genetically encoded biosensors of cytoplasmic and mitochondrial H₂O₂ in the genome of induced pluripotent stem cell (iPSC) li

SUBMITTER: Ustyantseva E 

PROVIDER: S-EPMC9142597 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature