Label-free multimodal nonlinear optical imaging of needle biopsy cores for intraoperative cancer diagnosis.

ABSTRACT:

Significance

Needle biopsy (NB) procedures are important for the initial diagnosis of many types of cancer. However, the possibility of NB specimens being unable to provide diagnostic information, (i.e., non-diagnostic sampling) and the time-consuming histological evaluation process can cause delays in diagnoses that affect patient care.

Aim

We aim to demonstrate the advantages of this label-free multimodal nonlinear optical imaging (NLOI) technique as a non-destructive point-of-procedure evaluation method for NB tissue cores, for the visualization and characterization of the tissue microenvironment.

Approach

A portable, label-free, multimodal NLOI system combined second-harmonic generation (SHG) and third-harmonic generation and two- and three-photon autofluorescence

SUBMITTER: Yang L 

PROVIDER: S-EPMC9142840 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature