Structure-Function Relationship Studies of Multidomain Levansucrases from Leuconostocaceae Family.

ABSTRACT: Levansucrase LevS from Leuconostoc mesenteroides B-512F is a multidomain fructansucrase (MD-FN) that contains additional domains (ADs) to the catalytic domain. However, the understanding of the effect that these ADs have on enzyme activity remains vague. To this aim, structure-function relationship studies of these LevS ADs were performed by evaluating both biochemical properties and the enzymatic capacity of truncated versions of LevS. Joint participation of the N- and C-terminal domains is essential for stability, activity, specificity, and polymerization processes. Specifically, the N-terminal region is involved in stability, while the transition region plays an essential role in the transfructosylation reaction and polymer elongation. Based on our results, we suggest that ADs interact

SUBMITTER: Garcia-Paz FM 

PROVIDER: S-EPMC9142893 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature