Phage ImmunoPrecipitation Sequencing (PhIP-Seq): The Promise of High Throughput Serology.

ABSTRACT: Phage ImmunoPrecipitation Sequencing (PhIP-Seq) is a high throughput serological technology that is revolutionizing the manner in which we track antibody profiles. In this review, we mainly focus on its application to viral infectious diseases. Through the pull-down of patient antibodies using peptide-tile-expressing T7 bacteriophages and detection using next-generation sequencing (NGS), PhIP-Seq allows the determination of antibody repertoires against peptide targets from hundreds of proteins and pathogens. It differs from conventional serological techniques in that PhIP-Seq does not require protein expression and purification. It also allows for the testing of many samples against the whole virome. PhIP-Seq has been successfully applied in many infectious disease investigations concernin

SUBMITTER: Tiu CK 

PROVIDER: S-EPMC9143919 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature