Spontaneous DNA Synapsis by Forming Noncanonical Intermolecular Structures.

ABSTRACT: We report the spontaneous formation of DNA-DNA junctions in solution in the absence of proteins visualised using atomic force microscopy. The synapsis position fits with potential G-quadruplex (G4) sites. In contrast to the Holliday structure, these conjugates have an affinity for G4 antibodies. Molecular modelling was used to elucidate the possible G4/IM-synaptic complex structures. Our results indicate a new role of the intermolecular noncanonical structures in chromatin architecture and genomic rearrangement.

SUBMITTER: Severov V 

PROVIDER: S-EPMC9144187 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature