Towards Unbiased Evaluation of Ionization Performance in LC-HRMS Metabolomics Method Development.

ABSTRACT: As metabolomics increasingly finds its way from basic science into applied and regulatory environments, analytical demands on nontargeted mass spectrometric detection methods continue to rise. In addition to improved chemical comprehensiveness, current developments aim at enhanced robustness and repeatability to allow long-term, inter-study, and meta-analyses. Comprehensive metabolomics relies on electrospray ionization (ESI) as the most versatile ionization technique, and recent liquid chromatography-high resolution mass spectrometry (LC-HRMS) instrumentation continues to overcome technical limitations that have hindered the adoption of ESI for applications in the past. Still, developing and standardizing nontargeted ESI methods and instrumental setups remains costly in terms of time and

SUBMITTER: Jaeger C 

PROVIDER: S-EPMC9144264 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature