Metabolic Profiling of the Hypothalamus of Mice during Short-Term Food Deprivation.

ABSTRACT: Nutrient availability and utilization in hypothalamic cells are directly associated with the regulation of whole-body energy homeostasis. Thus, establishing metabolic profiling in the hypothalamus in response to metabolic shift is valuable to better understand the underlying mechanism of appetite regulation. In the present study, we evaluate the alteration of lipophilic and hydrophilic metabolites in both the hypothalamus and serum of fasted mice. Fasted mice displayed an elevated ketone body and decreased lactate levels in the hypothalamus. In support of the metabolite data, we further confirmed that short-term food deprivation resulted in the altered expression of genes involved in cellular metabolic processes, including the shuttling of fuel sources and the production of monocarboxylate

SUBMITTER: Kim YJ 

PROVIDER: S-EPMC9144291 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature