Generated Randomly and Selected Functionally? The Nature of Enterovirus Recombination.

ABSTRACT: Genetic recombination in RNA viruses is an important evolutionary mechanism. It contributes to population diversity, host/tissue adaptation, and compromises vaccine efficacy. Both the molecular mechanism and initial products of recombination are relatively poorly understood. We used an established poliovirus-based in vitro recombination assay to investigate the roles of sequence identity and RNA structure, implicated or inferred from an analysis of circulating recombinant viruses, in the process. In addition, we used next-generation sequencing to investigate the early products of recombination after cellular coinfection with different poliovirus serotypes. In independent studies, we find no evidence for a role for RNA identity or structure in determining recombination junctions location. I

SUBMITTER: Alnaji FG 

PROVIDER: S-EPMC9144335 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature