Application of CRISPR/Cas9 System for Efficient Gene Editing in Peanut.

ABSTRACT: Peanuts are an economically important crop cultivated worldwide. However, several limitations restrained its productivity, including biotic/abiotic stresses. CRISPR/Cas9-based gene-editing technology holds a promising approach to developing new crops with improved agronomic and nutritional traits. Its application has been successful in many important crops. However, the application of this technology in peanut research is limited, probably due to the lack of suitable constructs and protocols. In this study, two different constructs were generated to induce insertion/deletion mutations in the targeted gene for a loss of function study. The first construct harbors the regular gRNA scaffold, while the second construct has the extended scaffold plus terminator. The designed gRNA targeting the

SUBMITTER: Neelakandan AK 

PROVIDER: S-EPMC9144340 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature