Dataset Information

Stability of ZIF-8 Nanoparticles in Most Common Cell Culture Media.

ABSTRACT: Zeolite imidazolate framework-8 (ZIF-8) is a promising platform for drug delivery, and information regarding the stability of ZIF-8 nanoparticles in cell culture media is essential for proper interpretation of in vitro experimental results. In this work, we report a quantitative investigation of the ZIF-8 nanoparticle's stability in most common cell culture media. To this purpose, ZIF-8 nanoparticles containing sterically shielded nitroxide probes with high resistance to reduction were synthesized and studied using electron paramagnetic resonance (EPR). The degradation of ZIF-8 in cell media was monitored by tracking the cargo leakage. It was shown that nanoparticles degrade at least partially in all studied media, although the degree of cargo leakage varies widely. We found a strong correlation between the amount of escaped cargo and total concentration of amino acids in the environment. We also established the role of individual amino acids in ZIF-8 degradation. Finally, 2-methylimidazole preliminary dissolved in cell culture media partially inhibits the degradation of ZIF-8 nanoparticles. The guidelines for choosing the proper cell culture medium for the in vitro study of ZIF-8 nanoparticles have been formulated.

SUBMITTER: Spitsyna AS

PROVIDER: S-EPMC9144353 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Stability of ZIF-8 Nanoparticles in Most Common Cell Culture Media.

Spitsyna Anna S AS Poryvaev Artem S AS Sannikova Natalya E NE Yazikova Anastasiya A AA Kirilyuk Igor A IA Dobrynin Sergey A SA Chinak Olga A OA Fedin Matvey V MV Krumkacheva Olesya A OA

Molecules (Basel, Switzerland) 20220518 10

Zeolite imidazolate framework-8 (ZIF-8) is a promising platform for drug delivery, and information regarding the stability of ZIF-8 nanoparticles in cell culture media is essential for proper interpretation of in vitro experimental results. In this work, we report a quantitative investigation of the ZIF-8 nanoparticle's stability in most common cell culture media. To this purpose, ZIF-8 nanoparticles containing sterically shielded nitroxide probes with high resistance to reduction were synthesiz ...[more]

PMID: 35630717

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Project description:Study questionDo plastic laboratory consumables and cell culture media used in ART contain bisphenols?Summary answerThe majority of human embryo culture media assessed contained bisphenol S close to the nanomolar concentration range, while no release of bisphenols by plastic consumables was detected under routine conditions.What is known alreadyThe deleterious effect of the endocrine disruptor bisphenol A (BPA) on female fertility raised concerns regarding ART outcome. BPA was detected neither in media nor in the majority of plastic consumables used in ART; however, it might have already been replaced by its structural analogs, including bisphenol S (BPS).Study design, size, durationSeventeen plastic consumables and 18 cell culture and ART media were assessed for the presence of bisphenols.Participants/materials, setting, methodsTen different bisphenols (bisphenol A, S, AF, AP, B, C, E, F, P and Z) were measured using an isotopic dilution according to an on-line solid phase extraction/liquid chromatography/mass spectrometry method.Main results and the role of chanceWhile the plastic consumables did not release bisphenols under routine conditions, 16 of the 18 cell culture and ART media assessed contained BPS. Six media exhibited BPS concentrations higher than 1 nM and reached up to 6.7 nM (1693 ng/l).Large scale dataN/A.Limitations, reasons for cautionFurther studies are required to investigate a greater number of ART media to identify less potentially harmful ones, in terms of bisphenol content.Wider implications of the findingsAs BPS has already been reported to impair oocyte quality at nanomolar concentrations, its presence in ART media, at a similar concentration range, could contribute to a decrease in the ART success rate. Thus far, there has been no regulation of these compounds in the ART context.Study funding/competing interestsThis study was financially supported by the 'Centre-Val de Loire' Region (Bemol project, APR IR 2017), INRAE, BRGM, the French National Research Agency (project ANR-18-CE34-0011-01 MAMBO) and the BioMedicine Agency (Project 18AMP006 FertiPhenol). The authors declare that they have no conflict of interest that could be perceived as prejudicing the impartiality of the reported research.

Dataset Information

Stability of ZIF-8 Nanoparticles in Most Common Cell Culture Media.

Publications

Stability of ZIF-8 Nanoparticles in Most Common Cell Culture Media.

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