Comparative diversity of ammonia oxidizer 16S rRNA gene sequences in native, tilled, and successional soils.

ABSTRACT: Autotrophic ammonia oxidizer (AAO) populations in soils from native, tilled, and successional treatments at the Kellogg Biological Station Long-Term Ecological Research site in southwestern Michigan were compared to assess effects of disturbance on these bacteria. N fertilization effects on AAO populations were also evaluated with soils from fertilized microplots within the successional treatments. Population structures were characterized by PCR amplification of microbial community DNA with group-specific 16S rRNA gene (rDNA) primers, cloning of PCR products and clone hybridizations with group-specific probes, phylogenetic analysis of partial 16S rDNA sequences, and denaturing gradient gel electrophoresis (DGGE) analysis. Population sizes were estimated by using most-probable-number (MPN)

SUBMITTER: Bruns MA 

PROVIDER: S-EPMC91447 | biostudies-literature | 1999 Jul

REPOSITORIES: biostudies-literature