Development of a Promising ¹⁸F-Radiotracer for PET Imaging Legumain Activity In Vivo.

ABSTRACT: Legumain has been found overexpressed in several cancers, which serves as an important biomarker for cancer diagnosis. In this research, a novel fluorine-18 labeled radioactive tracer [¹⁸F]SF-AAN targeting legumain was designed and synthesized for positron emission tomography (PET) imaging. Nonradioactive probe [¹⁹F]SF-AAN was obtained through chemical and solid phase peptide synthesis. After a simple one-step ¹⁸F labeling, the radiotracer [¹⁸F]SF-AAN was obtained with a high radiochemical conversion rate (>85%) and radiochemical purity (99%) as well as high molar activity (12.77 ± 0.50 MBq/nmol). The targeting specificity of [¹⁸F]SF-AAN for detecting legumain activity was investigated systematically in v

SUBMITTER: Lu C 

PROVIDER: S-EPMC9145320 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature