Examination of Genetic Control Elements in the Phototrophic Firmicute Heliomicrobium modesticaldum.

ABSTRACT: Heliomicrobium modesticaldum has been used as a model organism for the Heliobacteria, the only phototrophic family in the Firmicutes. It is a moderately thermophilic anoxygenic phototrophic bacterium that is capable of fermentative growth in the dark. The genetic manipulation of H. modesticaldum is still in its infancy. Methods to introduce genes through the use of exogenous plasmids and to delete genes from the chromosome through the use of the native CRISPR/Cas system have been developed in the last several years. To expand our genetic toolkit, it was necessary to control gene expression. In this study, we analyzed constitutive and inducible promoters developed for clostridia for their use in H. modesticaldum and further tested two reporters, adhB and lacZ<

SUBMITTER: Layton AM 

PROVIDER: S-EPMC9145376 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature