First Report on Genome Editing via Ribonucleoprotein (RNP) in Castanea sativa Mill.

ABSTRACT: Castanea sativa is an important tree nut species worldwide, highly appreciated for its multifunctional role, in particular for timber and nut production. Nowadays, new strategies are needed to achieve plant resilience to diseases, climate change, higher yields, and nutritional quality. Among the new plant breeding techniques (NPBTs), the CRISPR/Cas9 system represents a powerful tool to improve plant breeding in a short time and inexpensive way. In addition, the CRISPR/Cas9 construct can be delivered into the cells in the form of ribonucleoproteins (RNPs), avoiding the integration of exogenous DNA (GMO-free) through protoplast technology that represents an interesting material for gene editing thanks to the highly permeable membrane to DNA. In the present study, we developed the firs

SUBMITTER: Pavese V 

PROVIDER: S-EPMC9145500 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature