Characterization of an Immortalized Human Microglial Cell Line as a Tool for the Study of Diabetic Retinopathy.

ABSTRACT: The complexity of the retinal structure reflects on the difficulty to describe its composite cell interactions. Microglia is responsible for the immune reaction to inflammatory stimuli during diabetic retinopathy (DR), but most studies still use rodent cells. We characterized a commercially available immortalized human microglial line and tested its susceptibility to inflammation, to study the interactions between the neuro-vascular retinal portions in species-specific models. After checking the expression of microglial markers, we tried lipopolysaccharide (LPS) stimulation and several pro-inflammatory cocktails to select the best combination able to induce a significant M1 (inflammatory) response. We measured M1 induction through the expression of pro- and anti-inflammatory molecules and

SUBMITTER: Mazzeo A 

PROVIDER: S-EPMC9145666 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature