Identification and Molecular Characterization of the Operon Required for L-Asparagine Utilization in Corynebacterium glutamicum.

ABSTRACT: Understanding the metabolic pathways of amino acids and their regulation is important for the rational metabolic engineering of amino acid production. The catabolic pathways of L-asparagine and L-aspartate are composed of transporters for amino acid uptake and asparaginase and aspartase, which are involved in the sequential deamination to fumarate. However, knowledge of the catabolic genes for asparagine in bacteria of the Actinobacteria class has been limited. In this study, we identified and characterized the ans operon required for L-Asn catabolism in Corynebacterium glutamicum R. The operon consisted of genes encoding a transcriptional regulator (AnsR), asparaginase (AnsA2), aspartase (AspA2), and permease (AnsP). The enzymes and permease encoded in the operon were shown

SUBMITTER: Toyoda K 

PROVIDER: S-EPMC9145765 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature