Ammonia Concentration in the Eluent Influences Fragmentation Pattern of Triacylglycerols in Mass Spectrometry Analysis.

ABSTRACT: Correct assessment of the fatty acyl at the glycerol sn-2 position in triacylglycerol (TAG) analysis by liquid chromatography and mass spectrometry (LC-MS) is challenging. Ammonium hydroxide (NH₄OH) is the preferred choice for the solvent additive for the formation of the ammonium adduct ([M + NH₄]⁺). In this study, the influence of different NH₄OH concentrations in the eluents on TAG adduct formation and fragmentation under LC-MS analysis was assessed. Increasing NH₄OH concentrations delayed the chromatographic elution time according to a power function. The [M + NH₄]⁺ and [M + ACN + NH₄]⁺ adducts (where ACN means acetonitrile) were formed at all ammonium concentrations assayed. [M +

SUBMITTER: Velasco M 

PROVIDER: S-EPMC9146042 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature