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Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazole-Containing Compounds.

ABSTRACT: The naturally occurring dipeptide carnosine (β-alanyl-L-histidine) specifically attenuates tumor growth. Here, we ask whether other small imidazole-containing compounds also affect the viability of tumor cells without affecting non-malignant cells and whether the formation of histamine is involved. Patient-derived fibroblasts and glioblastoma cells were treated with carnosine, L-alanyl-L-histidine (LA-LH), β-alanyl-L-alanine, L-histidine, histamine, imidazole, β-alanine, and L-alanine. Cell viability was assessed by cell-based assays and microscopy. The intracellular release of L-histidine and formation of histamine was investigated by high-performance liquid chromatography coupled to mass spectrometry. Carnosine and LA-LH inhibited tumor cell growth with minor effects on fibroblasts, and L-histidine, histamine, and imidazole affected viability in both cell types. Compounds without the imidazole moiety did not diminish viability. In the presence of LA-LH but not in the presence of carnosine, a significant rise in intracellular amounts of histidine was detected in all cells. The formation of histamine was not detectable in the presence of carnosine, LA-LH, or histidine. In conclusion, the imidazole moiety of carnosine contributes to its anti-neoplastic effect, which is also seen in the presence of histidine and LA-LH. Despite the fact that histamine has a strong effect on cell viability, the formation of histamine is not responsible for the effects on the cell viability of carnosine, LA-LH, and histidine.

SUBMITTER: Seidel EC

PROVIDER: S-EPMC9146156 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazole-Containing Compounds.

Seidel Elisabeth Christiane EC Birkemeyer Claudia C Baran-Schmidt Rainer R Meixensberger Jürgen J Oppermann Henry H Gaunitz Frank F

International journal of molecular sciences 20220523 10

The naturally occurring dipeptide carnosine (β-alanyl-L-histidine) specifically attenuates tumor growth. Here, we ask whether other small imidazole-containing compounds also affect the viability of tumor cells without affecting non-malignant cells and whether the formation of histamine is involved. Patient-derived fibroblasts and glioblastoma cells were treated with carnosine, L-alanyl-L-histidine (LA-LH), β-alanyl-L-alanine, L-histidine, histamine, imidazole, ...[more]

PMID: 35628643

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Dataset Information

Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazole-Containing Compounds.

Publications

Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazole-Containing Compounds.

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