Laboratory Validation of a Real-Time RT-PCR Assay for the Detection of Jamestown Canyon Virus.

ABSTRACT: The neuroinvasive disease caused by Jamestown Canyon virus (JCV) infection is rare. However, increasing incidence and widespread occurrence of the infection make JCV a growing public health concern. Presently, clinical diagnosis is achieved through serological testing, and mosquito pool surveillance requires virus isolation and identification. A rapid molecular detection test, such as real-time RT-PCR, for diagnosis and surveillance of JCV has not been widely utilized. To enhance testing and surveillance, here, we describe the development and validation of a real-time RT-PCR test for the detection of JCV RNA. Three primer and probe sets were evaluated for analytical sensitivity and specificity. One probe set, JCV132FAM, was found to be the most sensitive test detecting 7.2 genomic equivale

SUBMITTER: Hughes HR 

PROVIDER: S-EPMC9146205 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature