Evaluating Cefoperazone-Induced Gut Metabolic Functional Changes in MR1-Deficient Mice.

ABSTRACT: Mucosal-associated invariant T cells are activated following the recognition of bacterial antigens presented by the major histocompatibility complex class I-related molecule (MR1). Previous metagenomics data showed that MR1^-/- knock-out (KO) mice had distinct microbiota and displayed a resistance to Clostridioides difficile (CDI) colonization vs. wild-type (WT) mice. In the present study, LC/MS-based untargeted metabolomics are applied to evaluate the changes in metabolic activities, in accordance with the changes in gut microbiota caused by cefoperazone (Cef) treatment. Adult C57Bl/6J WT and MR1^-/- KO mice were given sterile drinking water or spiked with 0.5 mg/mL Cef ad libitum for five days. Fecal pellets were collected daily, and both small intestinal and c

SUBMITTER: Sun J 

PROVIDER: S-EPMC9146321 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature