In Situ Electroporation on PERFECT Filter for High-Efficiency and High-Viability Tumor Cell Labeling.

ABSTRACT: Labeling-assisted visualization is a powerful strategy to track circulating tumor cells (CTCs) for mechanism study (e.g., tumor metastasis). Due to the rarity of CTCs in the whole blood, efficient simultaneous enrichment and labeling of CTCs are needed. Hereby, novel in situ electroporation on a previously-developed micropore-arrayed filter (PERFECT filter) is proposed. Benefiting from the ultra-small-thickness and high-porosity of the filter plus high precision pore diameter, target rare tumor cells were enriched with less damage and uniform size distribution, contributing to enhanced molecular delivery efficiency and cell viability in the downstream electroporation. Various biomolecules (e.g., small molecule dyes, plasmids, and functional proteins) were used to verify this in situ electr

SUBMITTER: Hun T 

PROVIDER: S-EPMC9146625 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature