Mammalian Brain Ca²⁺ Channel Activity Transplanted into Xenopus laevis Oocytes.

ABSTRACT: Several mutations on neuronal voltage-gated Ca²⁺ channels (VGCC) have been shown to cause neurological disorders and contribute to the initiation of epileptic seizures, migraines, or cerebellar degeneration. Analysis of the functional consequences of these mutations mainly uses heterologously expressed mutated channels or transgenic mice which mimic these pathologies, since direct electrophysiological approaches on brain samples are not easily feasible. We demonstrate that mammalian voltage-gated Ca²⁺ channels from membrane preparation can be microtransplanted into Xenopus oocytes and can conserve their activity. This method, originally described to study the alteration of GABA receptors in human brain samples, allows the recording of the activity of membrane r

SUBMITTER: Rousset M 

PROVIDER: S-EPMC9146698 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature