Data Mining Identifies CCN2 and THBS1 as Biomarker Candidates for Cardiac Hypertrophy.

ABSTRACT: Cardiac hypertrophy is a condition that may contribute to the development of heart failure. In this study, we compare the gene-expression patterns of our in vitro stem-cell-based cardiac hypertrophy model with the gene expression of biopsies collected from hypertrophic human hearts. Twenty-five differentially expressed genes (DEGs) from both groups were identified and the expression of selected corresponding secreted proteins were validated using ELISA and Western blot. Several biomarkers, including CCN2, THBS1, NPPA, and NPPB, were identified, which showed significant overexpressions in the hypertrophic samples in both the cardiac biopsies and in the endothelin-1-treated cells, both at gene and protein levels. The protein-interaction network analysis revealed <

SUBMITTER: Johansson M 

PROVIDER: S-EPMC9147176 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature