Evaluation of a Luminometric Cell Counting System in Context of Antimicrobial Photodynamic Inactivation.

ABSTRACT: Antimicrobial resistance belongs to the most demanding medical challenges, and antimicrobial photodynamic inactivation (aPDI) is considered a promising alternative to classical antibiotics. However, the pharmacologic characterization of novel compounds suitable for aPDI is a tedious and time-consuming task that usually requires preparation of bacterial cultures and counting of bacterial colonies. In this study, we established and utilized a luminescence-based microbial cell viability assay to analyze the aPDI effects of two porphyrin-based photosensitizers (TMPyP and THPTS) on several bacterial strains with antimicrobial resistance. We demonstrate that after adaptation of the protocol and initial calibration to every specific bacterial strain and photosensitizer, the luminometric method ca

SUBMITTER: Lehnig M 

PROVIDER: S-EPMC9147394 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature