Ustilago maydis Metabolic Characterization and Growth Quantification with a Genome-Scale Metabolic Model.

ABSTRACT: Ustilago maydis is an important plant pathogen that causes corn smut disease and serves as an effective biotechnological production host. The lack of a comprehensive metabolic overview hinders a full understanding of the organism's environmental adaptation and a full use of its metabolic potential. Here, we report the first genome-scale metabolic model (GSMM) of Ustilago maydis (iUma22) for the simulation of metabolic activities. iUma22 was reconstructed from sequencing and annotation using PathwayTools, and the biomass equation was derived from literature values and from the codon composition. The final model contains over 25% annotated genes (6909) in the sequenced genome. Substrate utilization was corrected by BIOLOG phenotype arrays, and exponential batch cultivations wer

SUBMITTER: Liebal UW 

PROVIDER: S-EPMC9147497 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature