Antigen Targeting of Porcine Skin DEC205⁺ Dendritic Cells.

ABSTRACT: Dendritic cell (DC) targeting by DEC205⁺ cells effectively promotes the internalization of antigens that may trigger a specific immune response. In this study, we evaluated the ability of a recombinant antibody, anti-DEC205 (rAb ZH9F7), to trigger cellular endocytosis in subpopulations of DCs and targeted cells after intradermal injection and subsequent migration toward lymph nodes. Furthermore, the cellular immune response was evaluated in pigs after intradermal application of the antigenized rAb ZH9F7 combined with porcine circovirus type 2 cap antigen (rAb ZH9F7-Cap). We demonstrated that rAb ZH9F7 recognized conventional type 1 and 2 DCs from the blood and skin and monocytes. It promoted receptor-mediated endocytosis and migration of cDCs and moDCs toward regional lymph node

SUBMITTER: Melgoza-Gonzalez EA 

PROVIDER: S-EPMC9147619 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature