Carbon dioxide fixation via production of succinic acid from glycerol in engineered Saccharomyces cerevisiae.

ABSTRACT:

Background

The microbial production of succinic acid (SA) from renewable carbon sources via the reverse TCA (rTCA) pathway is a process potentially accompanied by net-fixation of carbon dioxide (CO₂). Among reduced carbon sources, glycerol is particularly attractive since it allows a nearly twofold higher CO₂-fixation yield compared to sugars. Recently, we described an engineered Saccharomyces cerevisiae strain which allowed SA production in synthetic glycerol medium with a maximum yield of 0.23 Cmol Cmol^-1. The results of that previous study suggested that the glyoxylate cycle considerably contributed to SA accumulation in the respective strain. The current study aimed at improving the flux into the rTCA pathway accompanied by a higher CO₂

SUBMITTER: Malubhoy Z 

PROVIDER: S-EPMC9148483 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature