SEVA-Cpf1, a CRISPR-Cas12a vector for genome editing in cyanobacteria.

ABSTRACT:

Background

Cyanobacteria are photosynthetic autotrophs that have tremendous potential for fundamental research and industrial applications due to their high metabolic plasticity and ability to grow using CO₂ and sunlight. CRISPR technology using Cas9 and Cpf1 has been applied to different cyanobacteria for genome manipulations and metabolic engineering. Despite significant advances with genome editing in several cyanobacteria strains, the lack of proper genetic toolboxes is still a limiting factor compared to other model laboratory species. Among the limitations, it is essential to have versatile plasmids that could ease the benchwork when using CRISPR technology.

Results

In the present study, several CRISPR-Cpf1 vectors were developed for genetic manipulations in

SUBMITTER: Baldanta S 

PROVIDER: S-EPMC9148489 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature