Engineering Saccharomyces cerevisiae for production of the capsaicinoid nonivamide.

ABSTRACT:

Background

Capsaicinoids are produced by plants in the Capsicum genus and are the main reason for the pungency of chili pepper fruits. They are strong agonists of TRPV1 (the transient receptor potential cation channel subfamily V member 1) and used as active ingredients in pharmaceuticals for the treatment of pain. The use of bioengineered microorganisms in a fermentation process may be an efficient route for their preparation, as well as for the discovery of (bio-)synthetic capsaicinoids with improved or novel bioactivities.

Results

Saccharomyces cerevisiae was engineered to over-express a selection of amide-forming N-acyltransferase and CoA-ligase enzyme cascades using a combinatorial gene assembly method, and was screened for nonivamide production from supplemented vanill

SUBMITTER: Muratovska N 

PROVIDER: S-EPMC9148506 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature