Single-Cell RNA Sequencing of Bone Marrow Mesenchymal Stem Cells from the Elderly People.

ABSTRACT:

Background and objectives

Bone marrow mesenchymal stem cells (BMSCs) show considerable promise in regenerative medicine. Many studies demonstrated that BMSCs cultured in vitro were highly heterogeneous and composed of diverse cell subpopulations, which may be the basis of their multiple biological characteristics. However, the exact cell subpopulations that make up BMSCs are still unknown.

Methods and results

In this study, we used single-cell RNA sequencing (scRNA-Seq) to divide 6,514 BMSCs into three clusters. The number and corresponding proportion of cells in clusters 1 to 3 were 3,766 (57.81%), 1,720 (26.40%), and 1,028 (15.78%). The gene expression profile and function of the cells in the same cluster were similar. The vast majority of cells expressed the marker

SUBMITTER: Zhu D 

PROVIDER: S-EPMC9148839 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature