Measuring Protein Aggregation and Stability Using High-Throughput Biophysical Approaches.

ABSTRACT: Structure-function relationships of biological macromolecules, in particular proteins, provide crucial insights for fundamental biochemistry, medical research and early drug discovery. However, production of recombinant proteins, either for structure determination, functional studies, or to be used as biopharmaceutical products, is often hampered by their instability and propensity to aggregate in solution in vitro. Protein samples of poor quality are often associated with reduced reproducibility as well as high research and production expenses. Several biophysical methods are available for measuring protein aggregation and stability. Yet, discovering and developing means to improve protein behaviour and structure-function integrity remains a demanding task. Here, we discuss workflo

SUBMITTER: Kwan TOC 

PROVIDER: S-EPMC9149252 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature