A combined application of molecular docking technology and indirect ELISA for the serodiagnosis of bovine tuberculosis.

ABSTRACT:

Background

There is an urgent need to find reliable and rapid bovine tuberculosis (bTB) diagnostics in response to the rising prevalence of bTB worldwide. Toll-like receptor 2 (TLR2) recognizes components of bTB and initiates antigen-presenting cells to mediate humoral immunity. Evaluating the affinity of antigens with TLR2 can form the basis of a new method for the diagnosis of bTB based on humoral immunity.

Objectives

To develop a reliable and rapid strategy to improve diagnostic tools for bTB.

Methods

In this study, we expressed and purified the sixteen bTB-specific recombinant proteins in Escherichia coli. The two antigenic proteins, MPT70 and MPT83, which were most valuable for serological diagnosis of bTB were screened. Molecular docking technology was us

SUBMITTER: Song S 

PROVIDER: S-EPMC9149502 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature