Reference Gene Validation for RT-qPCR in PBMCs from Asthmatic Patients with or without Obesity.

ABSTRACT: Obesity is known to impair the efficacy of glucocorticoid medications for asthma control. Glucocorticoid-induced gene expression studies may be useful to discriminate those obese asthmatic patients who present a poor response to glucocorticoids. The expression of genes of interest is normalized with respect to reference genes (RGs). Ideally, RGs have a stable expression in different samples and are not affected by experimental conditions. The objective of this work was to analyze suitable RGs to study the role of glucocorticoid-induced genes in obese asthmatic patients in further research. The gene expression of eight potential RGs (GUSB, B2M, POLR2A, PPIA, ACTB, GAPDH, HPRT1, and TBP) was assessed with reverse transcription-quantitat

SUBMITTER: Bantula M 

PROVIDER: S-EPMC9149987 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature