Microbial Aerosols Generated from Standard Microbiological Laboratory Procedures.

ABSTRACT:

Background

Modern microbiology laboratories are designed to protect workers and the environment from microbial aerosols produced during microbiological procedures and accidents. However, there is only limited data available on the aerosols generated from common microbiology procedures.

Methods

A series of common microbiological procedures were undertaken with high concentration spore suspensions while air samplers were operated to sample the aerosols generated. Surface contamination from droplets was visualized using sodium fluorescein within the suspension. A total of 36 procedures were studied using different sample volumes (0.1-10 mL) and two spore suspension titers (10⁷ and 10⁹ colony forming units [cfu]/mL).

Results

The aerosol concentration

SUBMITTER: Pottage T 

PROVIDER: S-EPMC9150131 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature