3D genome organization in the epithelial-mesenchymal transition spectrum.

ABSTRACT:

Background

The plasticity along the epithelial-mesenchymal transition (EMT) spectrum has been shown to be regulated by various epigenetic repertoires. Emerging evidence of local chromatin conformation changes suggests that regulation of EMT may occur at a higher order of three-dimensional genome level.

Results

We perform Hi-C analysis and combine ChIP-seq data across cancer cell lines representing different EMT states. We demonstrate that the epithelial and mesenchymal genes are regulated distinctively. We find that EMT genes are regulated within their topologically associated domains (TADs), with only a subset of mesenchymal genes being influenced by A/B compartment switches, indicating topological remodeling is required in the transcriptional regulation of these genes. At

SUBMITTER: Pang QY 

PROVIDER: S-EPMC9150291 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature