Influence of DNA extraction kits on freshwater fungal DNA metabarcoding.

ABSTRACT:

Background

Environmental DNA (eDNA) metabarcoding is a common technique for efficient biodiversity monitoring, especially of microbes. Recently, the usefulness of aquatic eDNA in monitoring the diversity of both terrestrial and aquatic fungi has been suggested. In eDNA studies, different experimental factors, such as DNA extraction kits or methods, can affect the subsequent analyses and the results of DNA metabarcoding. However, few methodological studies have been carried out on eDNA of fungi, and little is known about how experimental procedures can affect the results of biodiversity analysis. In this study, we focused on the effect of DNA extraction method on fungal DNA metabarcoding using freshwater samples obtained from rivers and lakes.

Methods

DNA was extracted from f

SUBMITTER: Matsuoka S 

PROVIDER: S-EPMC9150701 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature