Crossing and selection of Chlamydomonas reinhardtii strains for biotechnological glycolate production.

ABSTRACT: As an alternative to chemical building blocks derived from algal biomass, the excretion of glycolate has been proposed. This process has been observed in green algae such as Chlamydomonas reinhardtii as a product of the photorespiratory pathway. Photorespiration generally occurs at low CO₂ and high O₂ concentrations, through the key enzyme RubisCO initiating the pathway via oxygenation of 1.5-ribulose-bisphosphate. In wild-type strains, photorespiration is usually suppressed in favour of carboxylation due to the cellular carbon concentrating mechanisms (CCMs) controlling the internal CO₂ concentration. Additionally, newly produced glycolate is directly metabolized in the C2 cycle. Therefore, both the CCMs and the C2 cycle are the key elements which limit th

SUBMITTER: Schad A 

PROVIDER: S-EPMC9151519 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature