Improved in-vivo airway gene transfer via magnetic-guidance, with protocol development informed by synchrotron imaging.

ABSTRACT: Gene vectors to treat cystic fibrosis lung disease should be targeted to the conducting airways, as peripheral lung transduction does not offer therapeutic benefit. Viral transduction efficiency is directly related to the vector residence time. However, delivered fluids such as gene vectors naturally spread to the alveoli during inspiration, and therapeutic particles of any form are rapidly cleared via mucociliary transit. Extending gene vector residence time within the conducting airways is important, but hard to achieve. Gene vector conjugated magnetic particles that can be guided to the conducting airway surfaces could improve regional targeting. Due to the challenges of in-vivo visualisation, the behaviour of such small magnetic particles on the airway surface in the presence of an app

SUBMITTER: Donnelley M 

PROVIDER: S-EPMC9151774 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature