Salmonella Typhimurium O-antigen and VisP play an important role in swarming and osmotic stress response during intracellular conditions.

ABSTRACT: Salmonella Typhimurium is a pathogen of clinical relevance and a model of study in host-pathogen interactions. The virulence and stress-related periplasmic protein VisP is important during S. Typhimurium pathogenesis. It supports bacteria invading host cells, surviving inside macrophages, swimming, and succeeding in murine colitis model, O-antigen assembly, and responding to cationic antimicrobial peptides. This study aimed to investigate the role of the O-antigen molecular ruler WzzST and the periplasmic protein VisP in swarming motility and osmotic stress response. Lambda red mutagenesis was performed to generate single and double mutants, followed by swarming motility, qRT-PCR, Western blot, and growth curves. Here we demonstrate that the deletion of visP affects swarming under osmotic

SUBMITTER: Manieri FZ 

PROVIDER: S-EPMC9151935 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature