AlphaFold 2 and NMR Spectroscopy: Partners to Understand Protein Structure, Dynamics and Function.

ABSTRACT: The artificial intelligence program AlphaFold 2 is revolutionizing the field of protein structure determination as it accurately predicts the 3D structure of two thirds of the human proteome. Its predictions can be used directly as structural models or indirectly as aids for experimental structure determination using X-ray crystallography, CryoEM or NMR spectroscopy. Nevertheless, AlphaFold 2 can neither afford insight into how proteins fold, nor can it determine protein stability or dynamics. Rare folds or minor alternative conformations are also not predicted by AlphaFold 2 and the program does not forecast the impact of post translational modifications, mutations or ligand binding. The remaining third of human proteome which is poorly predicted largely corresponds to intrinsically disor

SUBMITTER: Laurents DV 

PROVIDER: S-EPMC9152297 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature