Hepatocyte-Specific Knock-Out of Nfib Aggravates Hepatocellular Tumorigenesis via Enhancing Urea Cycle.

ABSTRACT: Nuclear Factor I B (NFIB) has been reported to promote tumor growth, metastasis, and liver regeneration, but its mechanism in liver cancer is not fully elucidated. The present study aims to reveal the role of NFIB in hepatocellular carcinogenesis. In our study, we constructed hepatocyte-specific NFIB gene knockout mice with CRISPR/Cas9 technology (Nfib^-/-; Alb-cre), and induced liver cancer mouse model by intraperitoneal injection of DEN/CCl₄. First, we found that Nfib^-/- mice developed more tumor nodules and had heavier livers than wild-type mice. H&E staining indicated that the liver histological severity of Nfib^-/- group was more serious than that of WT group. Then we found that the differentially expressed genes in the tumor tissue between Nf

SUBMITTER: Zhou L 

PROVIDER: S-EPMC9152321 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature