Characterization of a Novel Esterase Est33 From an Antarctic Bacterium: A Representative of a New Esterase Family.

ABSTRACT: Studies of microorganisms from extreme environments can sometimes reveal novel proteins with unique properties. Here, we identified a novel esterase gene (Est33) from an Antarctic bacterium. The protein was expressed and purified for biochemical characterizations. Site-mutation variants including S94A, D205A, and H233A were constructed to explore the structure-function relationship of the catalytic triad of Est33, and we found mutating Ser⁹⁴, Asp²⁰⁵, and His²³³ residues lead to a complete loss of enzyme activity. In addition, the catalytic Ser⁹⁴ located in a conserved pentapeptide motif GVSWG. Phylogenetic analysis showed that Est33 and its closely related homologs belonged to an independent group apart from other known family members, ind

SUBMITTER: Liu X 

PROVIDER: S-EPMC9152352 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature