The two-faced role of ATF2 on cisplatin response in gastric cancer depends on p53 context.

ABSTRACT:

Background

Activating transcription factor-2 (ATF2) is a member of the basic leucine zipper family of DNA-binding proteins, which exhibits both oncogenic and tumor suppression activity in different tumors. However, the molecular mechanism of its dual function in cancer chemotherapy especially in gastric cancer has still not been elucidated.

Methods

The protein expression and location of ATF2 in gastric cancer tissues was detected with immunohistochemistry assay, and the clinical significance was analyzed using TCGA and GEO database. The activation and impact of ATF2 in cisplatin treated cells were evaluated with western blot, incucyte live cell analysis, clone formation and tumor xenografts assays. Interaction between ATF2 and p53 was confirmed with immunoprecipitation and G

SUBMITTER: Xu L 

PROVIDER: S-EPMC9153165 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature