Metabolomic, proteomic and lactylated proteomic analyses indicate lactate plays important roles in maintaining energy and C:N homeostasis in Phaeodactylum tricornutum.

ABSTRACT:

Background

Phaeodactylum tricornutum accumulates lipids while the growth also increases under high CO₂, shedding light on its potential application in the reduction of CO₂ emissions and at the same time acquiring biodiesel raw materials. However, the sensing and transducing of high C:N signals and the related response mechanism(s) remained unknown.

Results

In this study, a multiple omics analysis was performed with P. tricornutum under low nitrogen (LN) and high CO₂ (HC) conditions. The results indicated that 2-oxoglutarate was significantly increased under both LN and HC. Meanwhile, proteins involved in carbon concentration mechanism decreased, indicated that 2-oxoglutarate might regulate C:N balance through suppressing carbon fixation. Lact

SUBMITTER: Huang A 

PROVIDER: S-EPMC9153171 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature